ROSE ELIXIR PURE formula is a unique complex, packed with key nutrients essential for healthy skin. ROSE ELIXIR PURE extract is an ideal mixture of Black Cumin, Rosa Centifolia and Aleppo Pine that act in synergy to fight acne problem and help nourishing and moisturizing the skin.

INCI: PINUS HALEPENSIS SEED EXTRACT (AND) CAPRYLIC/CAPRIC TRIGLYCERIDE (AND) NIGELLA SATIVA SEED EXTRACT (AND) ROSA CENTIFOLIA FLOWER EXTRACT.

CAS: 97435-13-7, 73398-61-5, 90064-32-7, 84604-12-6. EINECS: 306-894-1, 277-452-2, 290-094-1, 283-289-8

ORIGINE: Tunisia

Appearance: Yellow-Liquid oil.

Solubility: Soluble in oil & alcohol. Insoluble in water.

Use level: 0,1-1%

REGULATION: UE /USA /JAPAN Approved

STABILITY & STORAGE CONDITIONS: Stable for at least 2 years; Store in a cool, dry place.


Loaded with bioactive molecules that help repair and nourishes the skin with powerful anti-inflammatories properties.

Possess a powerful anti-inflammatory effect due to the high content of thymoquinone. All the plants used to formulate ROSE ELIXIR PURE possess an anti-acne potential besides many others effect such as antioxidant, anti-aging, moisturizing and anti-HEV/UVA which makes it a high quality ingredient. The presence of powerful natural, anti-inflammatory, antibacterial and antimicrobial in the marvelous ROSE ELIXIR PURE formula play a crucial role in keeping the skin look glowy thereby reducing inflammation and clogged pores, fighting acne and managing oily skin.

Inflammation is the immune system’s response to a damage caused in cells or tissues by bacterial pathogens or by any other biological, chemical, physical or mechanical aggressor. Even though painful, inflammation is usually a repair response.

The first step of inflammation is known as irritation. The stimulus or agent inducing this state of inflammation is an irritant compound. A big variety of materials are capable of causing an irritation response in the skin, including soaps, cosmetics, pesticides, organic dyes, solvents and industrial chemicals and waste.

Skin irritation includes different events leading to the development of an inflammatory response at the site of exposure. Cytokines are a family of proteins and glycoproteins that regulate the inflammatory and immune response, most of them are produced by epidermal cells. These cytokines are soluble molecules acting as chemical mediators released during the process, which help to intensify and propagate the inflammatory response; frequently including TNF-α, CCL2/MCP-1, C-RP, IL-1, IL-6, INF-γ, DUOX1, CXCR4 (SDF1 receptor), histamine, IL-10, TGF-β, COX-2, PGE2, etc. •Although its role in the inflammatory process is complex, these molecules modulate the activity and function of other cells to coordinate and control the inflammatory response. It has been suggested that cytokines and chemokines can communicate with sensory nerves through activation of high-affinity receptors, involved in pain and inflammation.

Tumor necrosis factor alpha (TNFα) is a cell signalling protein (cytokine) involved in systemic inflammation and is one of the cytokines that make up the acute phase reaction. Its primary role is in the regulation of immune cells, being able to induce fever, apoptotic cell death, cachexia, inflammation and to inhibit tumorigenesis, viral replication and response to sepsis via IL-1 and IL-6 producing cells. Interleukin 1 alpha (IL-1α) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. In general, Interleukin 1 is responsible for the production of inflammation, as well as the promotion of fever and sepsis. It is considered as a potent inflammatory cytokine that activates the inflammatory process, and its deregulated signaling causes devastating diseases manifested by severe acute or chronic inflammation. It is expressed as a precursor and is found in normal keratinocytes of the skin. Interleukin 6 (IL-6) is a potent inflammatory cytokine that exhibits functional pleiotropy in numerous cell types. It mediates several important physiological functions, most notably the control of the acute phase response at the beginning of acute inflammation, regulation of B-cell and T-cell differentiation and activation, and support of cell growth and survival. In addition, the encoded protein has been shown to be an endogenous pyrogen capable of inducing fever in people with autoimmune diseases or infections. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states.

IN VITRO ANALYSIS OF THE ANTI-INFLAMMATORY EFFECTS OF ELIXIR PURE Through gene expression analysis in human keratinocytes

Goal of the study: The assessment of anti-inflammatory potential through inhibition of the LPS-induced (Bacterial Lipopolysaccharide) expression of 3 genes (TNFα, IL-1α and IL-6), directly involved in inflammatory pathways, after in vitro treatment on human keratinocytes (HaCaT) through RTqPCR quantification. The inflammatory response will be induced by treatment with LPS (Bacterial Lipopolysaccharide), large molecules consisting of a lipid and a polysaccharide, found in the outer membrane of Gram-negative bacteria, able to elicit strong immune responses (Figure 1). To determined the non-toxic and effective concentrations, Cell viability was first assessed in Human HaCaT keratinocytes through quantification by MTT assay.

Methodology:

– HaCaT keratinocytes cells were treated with Elixir pure at 0.1 % during 24 hours. -After 30 minutes of treatment, Bacterial Lipopolysaccharide (LPS) was added at 100 ng/ml. -After 24 hours of incubation period, total RNA was purified, quantified and it was used to synthesize complementary DNA (cDNA).

– This cDNA from treated or untreated cells (control) was used to determine the relative gene expression of TNFα, IL-1α and IL-6through RT-qPCR. -β-ACT was used as reference gene. Data were statistically analyzed.

Results indicated that the treatment with ROSE ELIXIR PURE at 0.1 % significantly inhibited gene expression of TNFα, IL-1α and IL-6 by 36.5 ± 6.1 %, 30.5 ± 5.9 % and 41.1 ± 8.5 %, respectively, compared to the untreated control.

The in vitro treatment with ROSE ELIXIR PURE during 24 hours in human keratinocytes (HaCaT), displays anti-inflammatory effects, through significant reduction of Tumour Necrosis Factor alpha (TNFα), Interleukin 1 alpha (IL-1α) and Interleukin 6 (IL-6) gene expression, after induction with bacterial lipopolysaccharide (LPS), compared to the untreated control.